Protein Method Blocking Buffers
Santa Cruz Biotechnology BLOTTO NON-FAT DRY MILK
Blotto, Non-fat dry milk; Lyophilized powder; Contains Santa Cruz Biotechnology immunoanalytical grade blotto; Using w/phospho-specific antibodies: add 50mM NaF to Blotto to inhibit phosphatase activity
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 500MG
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
Teknova 10mM Tris-HCl with 0.1% Tween-20, pH 8.5. 1mL/Tube, 50 Pack, Sterile.
10mM Tris-HCl, 0.1% Tween-20
SCANTIBODIES LABORATORY INC Heterophilic Blocking Tube
This item must be ordered in multiples of 50 per supplier requirements
Tube, Blocking; Heterophilic; Unique blocking reagent composed of specific binders which inactivate heterophilic antibodies; Allows rapid and simple elimination of false positive heterophilic interference in plasma for sandwich immunoassays.
Boston Bioproducts Inc TBST 20X W TWEEN-20, 1% Tween-20, pH 7.4, Wash buffer, 4 x 1 L
TBST 20X W TWEEN-20, 1% Tween-20, pH 7.4, Wash buffer, 4 x 1 L
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 5 ML
UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 5 ML
Sigma Aldrich Fine Chemicals Biosciences Carbonate-Bicarbonate Buffer capsule | 50CAP
Carbonate-Bicarbonate Buffer capsule | 50CAP
LICOR Intercept T20 (TBS) Antibody Diluent (3 x 500)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Biotium 10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
Abcam Protein Block, 125ML
Protein block is a serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB). - Cited in over 90 publications - No need to match species for secondary antibodies, reducing animal serum handling - Ready-to-use – no mixing or dilution required
The product is subject to the following: Abcam Restricted Use Statement
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (20X, with 1% Tween-20, pH 7.4) - 1L
20X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
LICOR Intercept T20 (PBS) Antibody Diluent (3x500mL)
Intercept T20 (PBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects.The diluent contains Intercept (PBS) Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
TRIBIOSCIENCE INC 10% BSA Blocking PBS Solution, Sterile, 200 ML
Bovine Serum Albumin (BSA) Solution is formulated with bovine serum albumin Fraction V in 1x PBS (pH 7.2-7.6). It contains 2% bovine serum albumin Fraction V (20 mg/mL) in the buffer. The solution can be used for in vitro assays of molecular biology and protein assay.